Product Name :
PACT (PKR activating protein) / PRKRA Recombinant Rabbit Monoclonal Antibody [PSH02-14]
Predicted band size :
34 kDa
Observed band size :
34 kDa
Synonyms:
DYT16 antibody HSD14 antibody Interferon inducible double stranded RNA dependent protein kinase activator A antibody interferon-inducible double stranded RNA-dependent activator antibody Interferon-inducible double stranded RNA-dependent protein kinase activator A antibody PACT antibody PKR associated protein X antibody PKR associating protein X antibody PKR-associated protein X antibody PKR-associating protein X antibody 展开
Function :
Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at ‘Lys-386’ at ‘Ser-392’ respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Recombinant protein within human PACT aa 1-313 (O75569).
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P, IF-Cell, IF-Tissue, FC
Coupling :
unconjugated
Clone number:
PSH02-14
RRID:
AB_3072885
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Benzo[d]oxazole-7-carbaldehyde manufacturer Aliquot store at -20℃.1240587-95-4 Chemscene Avoid repeated freeze / thaw cycles.PMID:33634762
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 34 kDa
Positive control :
K-562cell lysate, HeLa cell lysate, MCF7 cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human testis tissue, 293T cell lysate, K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, Hela cell lysate, HL-60 cell lysate, mouse liver tissue lysate, rat skeletal muscle tissue lysate, rat brain tissue lysate, rat brain tissue, rat hippocampus tissue, 293T, PC-12.